Skip to main content
Figure 1 | BMC Immunology

Figure 1

From: Interplay between the heterotrimeric G-protein subunits Gαq and Gαi2 sets the threshold for chemotaxis and TCR activation

Figure 1

CXCL12-pretreatment attenuates TCR-induced Lck activation. (A) Jurkat TAg T cells were incubated with or without 10 &#956M PP2 for 30 minutes at 37°C prior to stimulation with CXCL12 (100 ng/ml) for the indicated time-points and stopped with lysis buffer. Cell lysates were subjected to immunoblotting with the indicated antibodies. (B) Jurkat TAg T cells were incubated with 100 ng/ml CXCL12 for 30 minutes at 37°C prior to stimulation with OKT3 (1.5 μg/ml) for the indicated time-points and stopped with lysis buffer. Cell lysates were subjected to immunoblotting with the indicated antibodies. Cells were also stained with anti-CXCR4 and surface expression of the receptor was analysed by flow cytometry. (C) Jurkat TAg T cells were transfected with Gαq-specific siRNA (GNAQ1103), Gαi2-specific siRNA (GNAI-2-1050) or control siRNA (GNAQ1103 M3). 48 hours post-transfection cells were stimulated with OKT3 (1.5 μg/ml) for the indicated time-points and stopped with lysis buffer. Cell lysates were subjected to immunoblotting with the indicated antibodies. All figures are representative of at least 3 independent experiments.

Back to article page