Figure 3

Effect of protease inhibitors on sCD226 level and mCD226 expression of normal PBMS or Jurkat cells. (A) Reduced levels of sCD226 in supernatant of PMA-stimulated PBMC or Jurkat cells treated with or without 1 mM protease inhibitor (1–10-Phenanathroline, AEBSF, or N-Ethylmaleimide). The data were the mean ± SD of three wells for each group. (*, P < 0.05 vs. nil group). (B) Enhanced expression of mCD226 on PMA-stimulated PBMC or Jurkat cells treated with or without 1 mM 1–10-Phenanathroline, AEBSF, or N-Ethylmaleimide. Solid line, without protease inhibitors; grey areas, with protease inhibitors; black areas, isotype controls. Representative experiments are shown (n = 3).