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Figure 4 | BMC Immunology

Figure 4

From: The expression of the β-defensins hBD-2 and hBD-3 is differentially regulated by NF-κB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis

Figure 4

C. albicans activate NF-κB in OE21 cells. OE21 cells were stimulated with A) 0.5 × 104 C. albican/ml, 1 × 105 C. albican/ml, or B) 0.5 × 106 PMNs/ml alone or with direct contact with 0.5 × 106 PMNs/ml and coincubation with 0.5 × 104 C. albican/ml in a transwell setting for indicated time periods. Nuclear extracts of these cells were submitted to EMSA using a radiolabelled probe for NF-κB. The arrow indicates the p50/p65 heterodimer. C) Supershift experiments with antibodies directed against the p50 or p65 subunit of NF-κB on nuclear extracts of OE21 cells treated for 1 h with 1 × 105 C. albican/ml.

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BMC Immunology

ISSN: 1471-2172

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