Skip to main content
Figure 6 | BMC Immunology

Figure 6

From: The expression of the β-defensins hBD-2 and hBD-3 is differentially regulated by NF-κB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis

Figure 6

Role of AP-1 and the MAPK Pathway in C. albicans mediated hBD induction. OE21 cells were stimulated with A) 0.5 × 104 C. albican/ml, 1 × 105 C. albican/ml, or B) 0.5 × 106PMNs/ml alone or with direct contact with 0.5 × 106 PMNs/ml and coincubation with 0.5 × 104 C. albican/ml in a transwell setting for indicated time periods. Nuclear extracts of these cells were submitted to EMSA using a radiolabelled probe for AP-1. The arrow indicates the AP-1 complex. C) Supershift experiments with an antibody directed against the c-Jun subunit of AP-1 on nuclear extracts of OE21 cells treated for 1 h with 1 × 105 C. albican/ml.

Back to article page