Figure 3
From: Conserved cryptic recombination signals in Vκ gene segments are cleaved in small pre-B cells
Vκ cRS are conserved at the DNA level. Using the Shannon entropy, the observed and maximum possible nucleotide diversity was calculated for each Vκ nucleotide position. Results for nucleotide positions occupying codon position 3 in FR1 and CDR1 are shown in panel A. Observed diversities are represented as bars, and maximum predicted diversities are represented by asterisks. The FR1-CDR1 boundary is marked by a vertical line. We computed the average difference between the observed and maximum possible DNA diversities for all nucleotide positions within codons that overlap one of the three conserved cRS (
) and for all nucleotide positions in FR codons not overlapping a cRS (
). We simulated the null distribution for - ; a histogram of 5000 simulated differences is shown in B. Difference is shown on the X-axis, and frequency is shown on the Y axis. The observed difference (0.073) is indicated by the vertical line.