Figure 2
From: Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts
Subfractionation of < 10 kDa whey ultrafiltrate by successive RP-HPLC (A, left panel 1 to 4), showing specific chemoattractant activity (A, medium and right panels) with B-cell lymphoblasts and MLN lymphocytes. Each fraction was collected and freeze-dried to remove any residual solvent. Chemoattractant activity was determined at 1/100 (black) and at 1/1000 (shaded) dilutions of the F1 and F2 fractions. The results are the mean number (± SEM) of specific migrating cells in independent experiments (* p < 0.05 versus spontaneous migration). Lymphoblast migration towards 2B1 fraction and subfractions at a dilution of 1/1000 (B), in the Boyden's chamber assay (Magnitude ×100). F2B1-A fraction and positive control (fMLP) induced potent chemotaxis. Nearly only the pores (8 μM) in the filter are seen in spontaneous migration (SM) and in F2B1-D fraction.