Figure 7
From: Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts
Calcium mobilization in myeloid cells expressing FPR, FPRL1 or FPRL2. Differentiated HL-60 cells and undifferentiated FPRL2-expressing HL-60 cells were loaded with 2 μM Fura-2 and analyzed with respect to calcium mobilization mediated by the addition of peptides. (A) Addition of the 2,7 kDa fragment from SAA2 (1 μM) to differentiated HL-60 cells was unable to mobilize intracellular calcium whereas 100 nM of recombinant of human ApoSAA, an agonist of FPRL1, induced a robust calcium rise (B) Undifferentiated HL-60 cells that stably express FPRL2 were unable to mobilize intracellular calcium in response to the addition of 1 μM of the 2,7 kDa fragment from SAA2 whereas a calcium rise was observed upon addition of 100 nM of WKYMVm, an agonist of FPRL2.