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Table 2 Checkerboard assay of lymphoblast migrationa towards 2.7 and 1 kDa peptides.

From: Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts

 

Concentration of the 2.7 kDa peptide (nM) above the filter

Concentration of the 2.7 kDa peptide (nM) below the filter

0

3.7

37

370

0

100.3 b ± 11.2

148.6 ± 14.2c

77.6 ± 1.7c

136.0 ± 9.8c

3.7

224.3 ± 2.0c

132.0 ± 8.0

114.7 ± 0.3c

156.5 ± 7.5

37

228.0 ± 12.7c

125.5 ± 13.5

146.0 ± 11.0

125.0 ± 12.0

370

119.0 ± 8.7

87.6 ± 3.2c

110.5 ± 0.5

74.3 ± 4.1

 

Concentration of the 1 kDa peptide (nM) above the filter

Concentration of the 1 kDa peptide (nM) below the filter

0

3.7

37

370

0

103 b .0 ± 5.2

103.3 ± 16.8c

124.7 ± 8.9

124.6 ± 9.5

3.7

49.0 ± 4.0

70.7 ± 4.2

85 ± 13.3

121.3 ± 39.6

37

64.0 ± 43.1

66.3 ± 8.9

60.7 ± 3.2

147.3 ± 6.8

370

64.3 ± 6.1c

49.0 ± 2.9c

74.7 ± 11.6c

103.3 ± 4.41

  1. aMigration was assessed with B lymphoblasts after 1 h of incubation in the presence of peptides in the upper, lower or both compartments. For underlined values, there was no gradient of peptide concentration between the upper and the lower compartments. In each column, above the underlined values, the peptide concentrations were lower than in the upper compartment and below the underlined values, the peptide concentrations were higher than in the upper compartment. Results are means ± SEM of migrating cells, for experiments carried out in triplicate. bSpontaneous migration in the absence of peptide. cSignificant difference between migration in the absence of a gradient (underlined values) and peptide-induced migration (p < 0.05 by Student's t test). A polycarbonate filter with 12 μm pores separated the two compartments of the chamber.