Figure 2
From: Sequence determinants of innate immune activation by short interfering RNAs
Innate immune stimulation of PBMCs by 207 siRNAs. Supernatant from PBMCs treated with 100 nM DOTAP complexed siRNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. Each point represents the mean of triplicate samples. (A) Frequency histogram of replicon inhibition by all 207 siRNAs in 5% bins. (B) Correlation between replicon inhibition an d free energy of hybridisation (ΔG) for all 207 siRNAs. (C) Correlation between replicon inhibition and U content for the strand of the duplex with higher U content (or both strands for duplexes with equal U content in both strands). (D) Correlation between replicon inhibition and U+G content for the strand of the duplex with higher U+G content (or both strands for duplexes with equal U+G content in both strands).