Innate immune stimulation of PBMCs and pDCs by siRNAs incorporating predicted TLR7/8 stimulatory motifs. Supernatant from PBMCs (left panel) or pDCs (right panel) treated with 100 nM DOTAP complexed siRNAs (single-stranded U rich strands = Stimulatory; annealed duplexes = Annealed) for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. Data is expressed as replicon expression on a log scale to allow visualisation of differences between highly active RNA species. Each point represents the mean of triplicate samples.