Figure 6
From: Sequence determinants of innate immune activation by short interfering RNAs
Innate immune stimulation of PBMCs and pDCs by ssRNAs incorporating predicted TLR7/8 stimulatory motifs. Supernatant from PBMCs (left panel) or pDCs (right panel) treated with 100 nM DOTAP complexed single-stranded U-rich RNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. Each point represents the mean of triplicate samples.