Innate immune stimulation of PBMCs by LNA modified siRNAs. Supernatant from PBMCs treated with 100 nM DOTAP complexed siRNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. LNA (red) indicates that the non-stimulatory strand contained LNA modifications at positions hybridizing to 4, 5, 8, 9, 11, 12, 15 and 16 of the stimulatory strand. LNA-5'3' (blue) indicates that the non-stimulatory strand contained LNA modifications at positions hybridizing to 1–4, and 16–19 of the stimulatory strand. Each point represents the mean of triplicate samples. Error bars indicate standard deviation.