Figure 8
From: Sequence determinants of innate immune activation by short interfering RNAs
Innate immune stimulation of PBMCs by LNA modified siRNAs. Supernatant from PBMCs treated with 100 nM DOTAP complexed siRNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. LNA (red) indicates that the non-stimulatory strand contained LNA modifications at positions hybridizing to 4, 5, 8, 9, 11, 12, 15 and 16 of the stimulatory strand. LNA-5'3' (blue) indicates that the non-stimulatory strand contained LNA modifications at positions hybridizing to 1–4, and 16–19 of the stimulatory strand. Each point represents the mean of triplicate samples. Error bars indicate standard deviation.