Innate immune stimulation of pDCs and TNFα production by PBMCs in response to duplex siRNAs. (A) Supernatant from pDCs treated with 100 nM DOTAP complexed siRNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. (B) Supernatant from PBMCs treated with 100 nM DOTAP complexed siRNAs for 24 hours was assayed for TNFα by ELISA. (C) Correlation between data in (A) and (B). Each point represents the mean of triplicate samples. Error bars indicate standard deviation.