Figure 4

2D-DIGE analysis of enriched SL of PHA blasts from two different donors. A. Fifty μg of fraction 2 protein were labeled with Cy3 (donor 1, green channel) or Cy5 (donor 2, red channel), respectively and spiked with unlabelled protein. Yellow indicates comparable abundance of proteins in both samples. One representative experiment of two performed with unseparated PHA blasts is shown – more experiments were performed with lymphocytes subpopulations. B. Statistical representation of the overall protein distribution in individual samples. The red line indicates the number of spots with a specific abundance ratio (log scale). The blue line represents the calculated Gaussian distribution. Red dots represent decreased and blue increased protein content in individual spots detected in both samples (considered from Cy3 → Cy5). The threshold was set to 1.7. C, D. Individual images corresponding to the individual samples of donor 1 (C) and donor 2 (D), respectively. Differential spots are marked either red or blue reflecting the color-coding in B. The numbers displayed in B and C correspond to the numbers in Figure 5 and table 1.