Figure 1

CD8⁺ T cell responses after priming with a DNA vaccine encoding DEC-205 targeted ovalbumin and boosting with an adenoviral vector. Mice were immunized with the indicated plasmids prior to boosting with adenoviral vectors encoding ovalbumin (Ad-Ova) or GFP (Ad-GFP). Unless stated otherwise, a DNA dose of 50 μg was used. One week after the boost, the percentage of SIINFEKL/H-2K^b tetramer positive cells (A), IFN-γ and CD107a (B, C) or IFN-γ and IL-2 (D) double-positive cells after stimulation with the SIINFEKL peptide were determined. A, B) Mean percentages with SEM of three independent experiments with a total of eight mice/group are shown. Significant differences (Bonferroni Multiple Comparison test) between groups primed with different DNA vaccines and boosted with Ad-OVA are marked (* p < 0.05; ** p < 0.01; *** p < 0.001). C, D) Dose response curve for DEC-Ova priming. The percentage of IFN-γ and CD107a positive (C) and IFN-γ and IL-2 positive (D) cells of CD8⁺ lymphocytes is shown for individual mice.