Figure 6
Half-Life and Signal Stability of Transferred T-lux Cells In Vivo. (A) T-lux T cells (3.5 × 106 cells) were adoptively transferred into congenic C57BL/6 (B6) mice (N = 8), and whole body bioluminescent imaging was obtained at the indicated times post-transfer. Control mice were given non-transgenic T cells; a representative day 5 image obtained from control mouse is shown to far right. (B) 3.5 × 106 T cells from CD45.2+ T-lux mice were adoptively transferred into congenic, CD45.1+ B6 mice and tissue-specific light emission quantitated. Light emission was calculated at the indicated times post-transfer for specific regions of interest (ROIs), including peripheral, cervical, and mesenteric (gut) lymph nodes. T-lux T cell numbers from recovered tissues were determined by flow cytometric quantitaton of CD45.2+ cells, and counts/sec per T cell were calculated. T-lux bioluminescent emission (counts/cell) within the indicated regions of interest is plotted over time. Error bars indicate SEM.