Figure 7
Clonal T cell Response to Antigen in vivo. Naïve CD4+ T cells were isolated from OT-II.T-lux transgenic mice (5 × 106) and transferred into congenic, WT B6 mice on day -2. Following baseline bioluminescent imaging on day -1, mice were given footpad injections of 100 μg or 300 μg of OVA peptide in ME adjuvant (red arrows; day 0). Control mice were given sham footpad injections containing adjuvant only (indicated as 0 μg OVA). Bioluminescence imaging was performed on all mice at the indicated time points. Representative images are shown for mice receiving 300 μg OVA peptide (A). The time of acquisition is shown above individual images ("d" indicates days). Bioluminescence emission (counts/sec) was quantified for all OVA doses and analyzed over time for various regions of interest. Shown in (B) are draining inguinal lymph node, non-draining inguinal lymph node, footpad injection site, and whole body. Error bars indicate SEM.