Table 2 Primers used to detect TCRγ rearrangement in cytokine-differentiated CD34-lineage- cells.
Primer | Sequence (5'-3') | Region | Orientation |
|---|---|---|---|
TCRL | TCCTACTGATCACTGTGCTG | Linker (L) region | F |
TCRL-IR | GGCCTTAAGTTGGCTGCATC | Â | R |
TCRL-ER | TGTTCTGTTGGAGATGGGAC | Â | R |
TCR 1/2 | CGCATATCATCTGTCAGAAC | Constant (C) region | F |
TCR 1/2IR | TACCACGTGTCACGTTGCTA | Â | R |
TCR 1/2ER | TGTCAACAATGAACCCGTTG | Â | R |
JγPF | AATCAAGGTATTTGGTCCCG | J cassette | F |
JγP1F | TACCACTGGTTGGTTCAAGA |  | F |
JγP1/2F | AAAGGGACTAGGCTCATAGT |  | F |
Jγ1/2F | AACAACACTTGTTGTCACAG |  | F |