Inhibition of nuclear translocation of transcription factors by ethanol. Mice were treated with ethanol at 6 g/kg (by gavage with a 32%, v/v solution). Thirty minutes later, LPS (60 μg/mouse) was administered intravenously. Then at the indicated times after LPS administration, peritoneal macrophages were isolated by lavage using ice cold PBS. Nuclear extracts were prepared and the indicated proteins assessed by western blot. Each lane represents a sample from one mouse (n = 2 for naive; n = 4 for most groups; n = 3 for LPS + ethanol at 60 min). Quantitation and statistical analysis of these data are shown in Figures 2 and 3.