Figure 5

CD4⁺CD25⁺CD45RO⁺ T cell frequency of an SLE patient before (A) and after (B) SLE diagnosis. (C) CD4+CD25+CD45RO+ T cell frequency of an SLE patient at the time of flare, (D) upon corticosteroid and anticoagulant therapy and (E) after IVIg administration. Lymphocytes gated according to forward and side scatter (FSC/SSC) from PBMC were screened by flow cytometry for the presence of CD4⁺CD25⁺CD45RO⁺ T cells. The FACS panel representing CD4⁺CD25⁺CD45RO⁺ is gated on CD4⁺ cells.