Asymmetric CD56 Expression in Vγ2Vδ2 T cells. IPP-stimulated cell lines were generated from 8 normal human donors and fractionated by magnetic bead separation into CD56+ and CD56- subsets. Vγ2 chains from each fraction were sequenced and translated. The frequency of each chain, identified by their unique CDR3 regions, with the fraction was calculated. Clonotypes found at a frequency below 3% were below the levels of detection for this assay, since below that cutoff we cannot reproducibly detect them. Clonotypes were considered committed to either the CD56+ or CD56- populations if the distribution was greater than 2:1. Black shading identifies clonotypes committed to CD56+ population, while those committed to the CD56- population are shaded in gray.