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Table 2 Mass Spectroscopy Identification of Proteins Enriched by Affinity Purification of Plasma with a CTLA4 Affinity Column and Analyzed by 2D-DIGE

From: Biochemical analysis of CTLA-4 immunoreactive material from human blood

Spot Fold Increase As32:MOPC Protein Name Accession No.
1 3.2 Plasminogen GI |38051823
2 1.9 Plasminogen GI |38051823
3 2.8 Plasminogen GI |38051823
4 3.5 Immunoglobulin heavy chain GI |10334573
5 4.8 Immunoglobulin heavy chain GI |10334573
6 2.1 hypothetical protein GI |34365168
7 1.9 hypothetical protein GI |34365168
8 5.7 This CDS feature is included GI 1684931
9 6.2 complement C1q subcomponent, α GI |54781353
10 5.0 complement C1q subcomponent, α GI |54781353
11 8.2 immunoglobulin lambda light chain C region GI |386813
12 2.4 Unknown (protein for MGC:31941) GI |18044245
13 5.4 Chain B, Globular Head Of The Complement System GI |38492827
14 2.2 anti HBs antibody light-chain Fab GI |11275326
15 2.4 α-Entamoeba histolytica immunoglobulin κ light chain GI |5360675
16 3.2 IGLC1 protein GI |20380868
17 2.2 anti HBs antibody light-chain Fab GI |11275326
  1. Plasma proteins that preferentially bound a to the CTLA4 specific antibody AS32 compared to the irrelevant control antibody MOPC were identified by 2D-DIGE. Spots were picked and identified by MALDI spectroscopy.