Biochemical analysis of CTLA-4 immunoreactive material from human blood

BMC Immunology

Table 2 Mass Spectroscopy Identification of Proteins Enriched by Affinity Purification of Plasma with a CTLA4 Affinity Column and Analyzed by 2D-DIGE

Spot	Fold Increase As32:MOPC	Protein Name	Accession No.
1	3.2	Plasminogen	GI \|38051823
2	1.9	Plasminogen	GI \|38051823
3	2.8	Plasminogen	GI \|38051823
4	3.5	Immunoglobulin heavy chain	GI \|10334573
5	4.8	Immunoglobulin heavy chain	GI \|10334573
6	2.1	hypothetical protein	GI \|34365168
7	1.9	hypothetical protein	GI \|34365168
8	5.7	This CDS feature is included	GI 1684931
9	6.2	complement C1q subcomponent, α	GI \|54781353
10	5.0	complement C1q subcomponent, α	GI \|54781353
11	8.2	immunoglobulin lambda light chain C region	GI \|386813
12	2.4	Unknown (protein for MGC:31941)	GI \|18044245
13	5.4	Chain B, Globular Head Of The Complement System	GI \|38492827
14	2.2	anti HBs antibody light-chain Fab	GI \|11275326
15	2.4	α-Entamoeba histolytica immunoglobulin κ light chain	GI \|5360675
16	3.2	IGLC1 protein	GI \|20380868
17	2.2	anti HBs antibody light-chain Fab	GI \|11275326

Plasma proteins that preferentially bound a to the CTLA4 specific antibody AS32 compared to the irrelevant control antibody MOPC were identified by 2D-DIGE. Spots were picked and identified by MALDI spectroscopy.

ISSN: 1471-2172