Figure 2From: Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screeningIdentification of FasL-interacting SH3 domains. Phagemid DNA from transduced clones was isolated and sequenced to unambiguously identify the SH3 domains responsible for interactions with glutathione S-transferase (GST) (white) and GST-hFasL(1-80) (black and grey), respectively. SH3 domains binding unspecifically to the GST moiety were identified by GST library panning. Data are shown from individual experiments with low (0.15% Tween-20, A) and high (0.5% Tween-20, B) detergent concentration. In B, dark and grey boxes show results from two individual panning rounds.Back to article page