Figure 3From: Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screeningFasL precipitates with the SH3 domain of Itk. Pull-down experiment with a GST-Itk SH3 domain fusion protein from whole cell lysates of stably FasL transfected KFL9 and JFL39.1 cells. Lysates were subjected to pull-down experiments with the indicated recombinant proteins. Precipitates were analyzed by SDS-PAGE and Western blot for the presence of FasL using the mAb clone G247-4.Back to article page