Figure 4

The FasL N-terminus precipitates sorting nexin. Pull-down experiment with GST fusion proteins containing the intracellular part of FasL from transiently transfected 293 T cells. Overexpression of sorting nexins in 293 T cells was tested 18 hours post transfection in whole cell lysates (upper panel). The corresponding lysates were subjected to precipitation with GST alone as a control or with a GST fusion protein containing the intracellular region of human FasL (hFasL(1-80)). Precipitated proteins were analyzed by SDS-PAGE and Western blotting using an anti-HA antibody. (EGFP, EGFP-transfected control cells; ctrl, vector-transfected controls).