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Figure 2 | BMC Immunology

Figure 2

From: Selenium deficiency impairs host innate immune response and induces susceptibility to Listeria monocytogenes infection

Figure 2

Analysis of splenic cell populations in L. monocytogenes infection. The populations of the NK cell subsets are indicated for the four groups; Group I, non-infected Se-adequate; Group II, non-infected Se-deficient; Group III, infected Se-adequate; and Group IV, infected Se-deficient. Cell surface expression of the indicated markers was determined for splenic lymphocytes from above four groups of mice on hour 72 of L. monocytogenes infection. Data are shown as percentage of positive cells in the spleen for each marker. Data are plotted as mean ± SD of analysis (50,000 events per sample) done on individual mouse spleen (N = 5 for each group). Statistical comparisons done between Se-adequate and Se-deficient groups indicated significant reduction in the expression of CD4 and CD8 under the conditions of Se deficiency. Statistical analysis done between Se-adequate and infected Se-adequate mice groups indicated significant increase in the expression of NK1.1 and DX5 post infection. Statistical analysis done between infected Se-adequate and infected Se-deficient mice groups indicated significant reductions in CD4+CD25+(A), TCRγδ+(B), CD8+(C), CD4+(D), B220+(E), F480+ and MAC-1+(F), CD11c+(G), Gr-1+(H), DX5+Ly49D+(I), DX5+CD94+(J), DX5+(K), and NK1.1+(L) populations under the conditions of Se deficiency. The percentages of DX5+Ly49D+(M), DX5+CD94+(N), DX5+(O), and NK1.1+(P) cells obtained after analysis of individual spleen samples were converted into numbers of each cell population based on the total splenocyte count obtained for each mouse. Results are expressed as the mean cell count per group ± standard deviation (SD). *p < 0.05, **p < 0.01.

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