Figure 1
From: Selenoproteins regulate macrophage invasiveness and extracellular matrix-related gene expression
Profiling of selenoprotein gene expression in macrophages. (A) Selenoprotein gene expression in macrophages (BMDMs) and fibroblasts (NIH3T3 embryonic fibroblasts) was analyzed by real-time qPCR and is shown as relative mRNA level to GUSB (β-glucuronidase; internal control). (B) Macrophages left unstimulated (None) and stimulated with LPS (100 ng/ml; 4 h) were subjected to RNA extraction and real-time qPCR. Data represent mean ± standard deviation (n = 4). *, p < 0.05. (C) 75Se-labeled selenoproteins in macrophages are visualized by autoradiography after SDS electrophoresis. Macrophages were left unstimulated (None) and stimulated with LPS (100 ng/ml; 6 h) before protein extraction. The identities of the major, labeled selenoproteins are designated on the right of panel. CBB, Coomassie Brilliant Blue. (D) Whole cell lysates from macrophages treated with LPS as in (C) were analyzed by immunoblotting with antibodies against the proteins indicated on the left. PS, Ponceau S.