Figure 2
From: Selenoproteins regulate macrophage invasiveness and extracellular matrix-related gene expression
Characterization of ΔTrspMmacrophages. (A) The levels of tRNA[Ser]Sec and tRNASerin macrophages (BMDMs) derived from control and ΔTrspMmice as determined by Northern blotting are shown. (B) 75Se-labeled selenoproteins are visualized by autoradiography after SDS electrophoresis as in Figure 1B. CBB, Coomassie Brilliant Blue. (C) ROS production was analyzed by confocal microscopy following DCFDA staining. Fluorescence (left panels) and phase contrast (right panels) images are shown. (D) ROS production was analyzed by flow cytometry following DCFDA staining.