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Figure 4 | BMC Immunology

Figure 4

From: Induction of granzyme B expression in T-cell receptor/CD28-stimulated human regulatory T cells is suppressed by inhibitors of the PI3K-mTOR pathway

Figure 4

Treg-enriched peripheral blood CD4+ T cells containing approximately 80% Tregs (Tr) and 20% Tconv (Tc) were evaluated for expression of CD4, FOXP3 and CD127 prior to expansion (panel A). Samples were next labeled with CFSE then expanded for six days with CD3/CD28 beads and IL-2. Subsequently, samples were simultaneously evaluated for FOXP3 expression and CFSE staining intensity by flow cytometry. Dot plots showing proliferation in the FOXP3+ and FOXP3- fractions are shown in panel B along with an overlay of histograms for CFSE staining (Tregs are shown in red and Tconv are shown in blue). As a control a CFSE-labeled but unstimulated sample is shown in black. The data is representative of three similar experiments from multiple donors.

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BMC Immunology

ISSN: 1471-2172

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