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Figure 3 | BMC Immunology

Figure 3

From: Chemokines are secreted by monocytes following OK-432 (lyophilized Streptococcus pyogenes) stimulation

Figure 3

OK-432 augments MCP-1 gene expression in MOs from healthy donors and HNSCC patients and correlates with MCP-1 protein levels upon inhibition with piceatannol and wortmannin. MCP-1 gene expression was assessed in MOs stimulated with OK-432 for 24 h from three randomly selected healthy donors (A) or two HNSCC patients on two separate days (C). In separate experiments, MOs, stimulated with piceatannol (120 μM) (B) or wortmannin (100 nM) (D) for 30 min followed by stimulation with OK-432 for an additional 24 h, were analyzed by ELISA or real-time PCR with respect to MCP-1 production and gene expression, respectively. A, B, and D: n = 3; C: n = 2.

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