Figure 8

Syk and PI-3 kinase-independent signaling for OK-432 stimulation of MOs. Piceatannol (1 μM) or diluent control was added to MO cultures for 30 min prior to addition of OK-432 for 24 h (A). LY294002 (50 μM) was added to MOs for 30 min prior to addition of OK-432 for 24 h (B). In separate experiments, a combination of LY294002 (50 μM) and piceatannol (30 μM) was added to whole blood and throughout the monocyte isolation procedure (C) and followed by stimulation with OK-432 or left unstimulated for 24 h (D). Supernatants were analyzed using Multiplex (MIP) or ELISA (MCP-1). A: n = 4; B: n = 5; C: n = 4; D: n = 4.