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Figure 5 | BMC Immunology

Figure 5

From: Plasmodium chabaudi limits early Nippostrongylus brasiliensis-induced pulmonary immune activation and Th2 polarization in co-infected mice

Figure 5

Western blot analysis of ChaFF protein expression in BALF of Nb, Pcc and co-infected mice. Lung lavages harvested at days 3, 5, 7 and 20 pi indicated that the ChaFF proteins RELMα (A) and Ym1 (B) exhibited temporal dynamics that were similar to those observed for mRNA expression in lung tissue (Fig. 4). In particular, expression of both proteins was significantly lower in the lungs of co-infected (Nb+Pcc) than in Nb mice at 7 days pi (RELMα P = 0.0067 and Ym1 P = 0.0104). This pattern had reversed by 20 days pi, with increased ChaFF protein expression in Nb+Pcc compared to Nb mice (RELMα P = 0.0398 and Ym1 P = 0.0171). Data are expressed as percentage of a positive control sample (pooled lung lavages of three Nb-infected mice). Bars indicate mean ± SEM of combined results of 2 independent experiments, each with 4-9 mice per group per timepoint. The symbol ¶ indicates significance of the difference between co-infected and Nb mice (P values included above and in Results text).

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