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Table 1 HLA-A*0201 binding affinity of candidate epitope peptides on T2 cells.

From: Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV

Peptide

Denomination

Start position

Sequence

Score*

FI

No.1

Sp1

2

FIFLLFLTL

24

0.5

No.2

Sp2

851

MIAAYTAAL

26

0.8

No.3

Sp3

404

VIADYNYKL

26

0.4

No.4

Sp4

208

DLPSGFNTL

24

0.1

No.5

Sp5

940

ALNTLVKQL

27

1.1

No.6

Sp6

1174

NLNESLIDL

27

1.1

No.7

Sp7

673

SIVAYTMSL

25

1.2

No.8

Sp8

958

VLNDILSRL

27

1.5

  1. * HLA-A*0201-binding motif score from algorithm SYFPEITHI ≥ 24. The eight peptides were also predicted and selected using ProPred1, in which the threshold of HLA-A2-binding motif is 4% and the threshold of proteasomal and immunoproteasomal cleavage site is 8%.
  2. † Increase of HLA-A*0201 molecules on T2 cell surface. FI = [(mean FITC fluorescence with the given peptide - mean FITC fluorescence without peptide)/(mean FITC fluorescence without peptide)]. FI > 1.0 indicates high-affinity peptides; FI ≤ 1.0, low-affinity peptides. HLA-A*0201-restricted peptide S411-420 was used as a positive control for HLA-A*0201-binding ability, while the H-2b-restricted peptide HBcAg(131-140) was used as a negative control.