Cytotoxicity of CD8+ T lymphocytes as measured by β-hexosamidase release. CD8+ T lymphocytes were stimulated to release cytotoxic granules by CD3 cross-linking (black columns). A non-cross-linking isotypic IgG2a antibody was used as control (IgG; white columns). The graph shows mean values and standard deviation of five independent experiments with cells from different donors. Asterisks mark statistically significant changes (p < 0.05).