Cell viability (A), Cell cytotoxicity (B) of Caco-2 and normal human FHs74-Int cells treated with undigested and digested Enprocal (Enprocal D) 100–8000 μg/ml of each for 48 hours. Cell viability was determined by trypan blue exclusion assay. Cell cytotoxicity was determined by LDH release assay. (C) Cell viability (trypan blue exclusion assay) of Caco-2 monolayers treated with Enprocal D (500–2000 μg/ml) and with other digested milk product controls. All treatments were performed in triplicate and assay was repeated three times independently with similar results. The mean for representative experiment was calculated and presented as a mean ± SD values. ** Indicates a highly significant P < 0.001 value from the control with media only. *Indicates a significant P < 0.05 value from the control with media only.