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Figure 3 | BMC Immunology

Figure 3

From: Gut health immunomodulatory and anti-inflammatory functions of gut enzyme digested high protein micro-nutrient dietary supplement-Enprocal

Figure 3

Cell cytotoxicity determined by LDH release assay (A) and cell death (apoptosis/necrosis) (B) following treatment with 500–2000 μg/ml of Enprocal D and other digested milk product controls. Cells were treated for 48 hours with milk products and stained by TUNEL analysis for apoptotic cells, Annexin-V-fluos analysis and counterstained with propidium iodide to reveal necrotic cells. Cell death is shown here in terms of apoptotic (A/I) and necrotic indices (N/I). All treatments were performed in triplicate and assay was repeated three times independently with similar results. The mean for representative experiment was calculated and presented as a mean ± SD values. ** Indicates a highly significant P < 0.001 value from the control with media only. *Indicates a significant P < 0.05 value from the control with media only.

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