Cell surface activation marker expression on the human THP-1 (PMA differentiated) macrophages for MHC-I and MHC-II molecules ( A ) and co-stimulatory (CD80, CD86 and CD40) molecules ( B ) is modulated by Enprocal D and compared with other digested milk product controls in vitro. Responses were measured by the upregulation of the surface markers CD80, CD86 and CD40. A negative control of cells without treatment is shown. Positive control of LPS and IFN-γ is shown. All treatments were performed in triplicate and assay was repeated three times independently with similar results. The mean for each experiment was calculated and presented as a mean ± SD values. ** Indicates a highly significant P < 0.001 value from the control with media only. *Indicates a significant P < 0.05 value from the control with media only.