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Table 2 Effect of Enprocal D and other digested milk product controls on macromolecular absorption determined by Horseradish Peroxidase (HRP) flux assay.

From: Gut health immunomodulatory and anti-inflammatory functions of gut enzyme digested high protein micro-nutrient dietary supplement-Enprocal

Ingredients 4 hr (Mean ± SD) 8 hr (Mean ± SD) 16 hr (Mean ± SD)
Enprocal 12.5 ± 1.8* 17.5 ± 1.3* 35.5 ± 1.6**
WPC 7.9 ± 0.74 15.6 ± 0.7* 22.2 ± 0.7**
P1 6.5 ± 0.8 10.9 ± 0.6 24.5 ± 0.7**
P2 10.5 ± 1.4 18.5 ± 1.2* 40.4 ± 1.3**
Heat SMP 8.5 ± 0.6 12.4 ± 0.5 18.4 ± 0.5**
No treatment 8.3 ± 1.3 8.5 ± 1.1 9.1 ± 2.6
Enzyme cocktail 8.5 ± 1.2 8.5 ± 1.2 8.7 ± 1.3
Triton-X 100 80.5 ± 2.8** 85.3 ± 2.5** 88.5 ± 2.6**
  1. HRP flux results are shown in percentage for 4–16 hours determined in the basolateral compartment. All samples were assayed in three independent assays each performed in triplicate and the mean for all experiments was calculated and presented as a mean ± SD values.
  2. ** Indicates a highly significant P < 0.001 value from the control with media only (no treatment).
  3. *Indicates a significant P < 0.05 value from the control with media only (no treatment). Triton-X 100 is a known cell disruptor and act as a positive control in HRP flux assay
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