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Figure 3 | BMC Immunology

Figure 3

From: AUF1 is involved in splenic follicular B cell maintenance

Figure 3

Flow cytometric analysis of lymphocyte populations in bone marrow chimeric mice. (a) Ten weeks post bone marrow transplantation, splenocytes from indicated bone marrow chimeric mice were stained with the indicated antibodies and analyzed. Numbers indicate the percentage of lymphoid cells in quadrants or enclosed within indicated gates. Contour plots gated for B220+ are indicated. FO B cells are represented by IgDhiIgMlo, CD21intCD24lo, and CD21intCD23hi gates and MZ B cells are represented by the CD21hiCD23lo gate. Splenocytes in bone marrow chimeras are of donor origin as shown by CD45 analysis. (b) Decreased CD23 expression in B cells (B220+) and FO B cells (IgMloIgDhi gated) from chimeric mice receiving bone marrow from AUF1-/- donors. Numbers in parenthesis indicate the MFI of CD23 expression.

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