Figure 6
From: AUF1 is involved in splenic follicular B cell maintenance
Normal in vitro maturation and BCR signaling in AUF1-deficient B cells. (a) Sorted T2 B cells (B220+CD21hiCD24hi) were cultured with (+) or without (-) F(ab')2 anti-IgM (10 μg/ml) for 48 h, and then analyzed by flow cytometry for CD21 and CD24 expression. (b) The percentage of live cells falling into T2 (CD21hiCD24hi) and FO (CD21intCD24lo) gates are shown; mean ± SD, n = 3. (c) Calcium flux analysis in response to BCR cross-linking. Arrow indicates time at which anti-IgM (10 μg/ml) was added to cells. (d) CD86 upregulation in B cells with (gray) or without (white) stimulation with F(ab')2 anti-IgM. Data in (c-d) is representative of three independent experiments. (e) mRNA levels of BAFF-R in sorted FO B cells (left panel) and BAFF in splenocytes (right panel) as determined by qRT-PCR.