Figure 4
Impaired B cell activation by BCR-crosslinking of offpring from immunized mothers. Neonate pups (3 d-o) were immunized with OVA and evaluated (20 d-o) for: (A) proliferative spleen cell response to OVA; (B) purified B-cell response for CpG oligodeoxynucleotide type B (5 μg/mL) stimuli; (C) purified B cell from nonimmunized mice incubated with F(ab')2 anti-mouse IgM Ab (50 μg/mL) in the presence of IgG from from OVA-immunized unrelated adult mice (10, 50, 100 μg/mL), or monoclonal antibody anti-OVA (α OVA, 5 μg/mL) plus OVA (10 μg/mL), incubated for 96 h [data expressed in Stimulation Index (S.I.)]; (D) cytokine measurements in the supernatants of spleen cell culture after 72 h of stimulation with OVA by cytometric bead array. The results represent the mean ± SEM of 9 mice per group. *P ≤ 0.05 compared to offspring from nonimmunized mothers.