Figure 5

Effect of passive IgG transference to neonates on B and T cell responses. Neonate pups (3 d-o) from nonimmunized mothers injected with IgG from nonimmunized or immunized mothers and simultaneously immunized with OVA were evaluated (20 d-o) for: (A) anti-OVA IgE Ab levels by PCA reaction; (B) B cell FcγRIIb expression (B220+IgM+) and histogram of FcγRIIb expression on B cells of offspring from immunized (shaded histogram, MFI in bold numbers) or nonimmunized mothers (white histogram, MFI in light numbers); (C) intracellular cytokines of splenic B cells (B220+) or (D) CD4+ T cells after 24 h incubation with 10 μg/mL brefeldin A; data shown in B-D were obtained flow cytometry. The results represent the mean ± SEM of 9 mice per group. * P ≤ 0.05 compared to offspring from nonimmunized mothers.