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Figure 8 | BMC Immunology

Figure 8

From: Gallin; an antimicrobial peptide member of a new avian defensin family, the ovodefensins, has been subject to recent gene duplication

Figure 8

Recombinant gallin identified by western blotting performed using a SuperSignal® West HisProbe™ Kit (Pierce, Rockford IL and visualised with Luminol/Enhancer solution with stable Peroxide Solution (Thermo, USA). The membrane was exposed to X-ray film for 30 seconds. The lanes show the signal from samples derived from either the supernatant or the bacterial pellet after induction by 1 mM IPTG for 0, 2, 3 5 or 15 hours. Molecular weight was determined by interpolation from a Spectra™ Multicolor Broad Range Protein Ladder (Fermentas) run in lane M.

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BMC Immunology

ISSN: 1471-2172

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