Figure 2

(A) DC viability after exposure to paclitaxel. Day 7 DC were incubated with paclitaxel at 1-100 μM for 2 h, washed and returned to culture for up to 120 h (5 days). Viability was assessed by trypan blue (and propidium iodide (PI) dye exclusion, data not shown). Representative data from one of 3 individual donors are shown. No deleterious effect on survival was seen, (B). DC were exposed to paclitaxel at 1-100 μM for 2, 24 or 48 h and viability assessed at the end of 48 h by PI dye exclusion. Short-term exposure of DC to paclitaxel had no effect on viability, however, 100 μM paclitaxel for 48 h did induce a significant loss in viability (* P < 0.05).