Figure 4

DC exposed to paclitaxel have less endocytic function shown by uptake of FITC-conjugated dextran. Control and paclitaxel treated DC were incubated with FITC-dextran for 2 h at either 37°C (black bars) or 4°C (white bars). DC were washed with ice cold PBS to remove unbound FITC-dextran prior to FACS analysis. Combined data from 3 separate donor experiments are shown. Increasing doses of paclitaxel significantly reduced active endocytosis compared to no taxol exposure (P < 0.05 with 10 μmol taxol exposure).