| | | Taxol(μM) |
---|
Marker
|
No Drug
|
LPS
|
1
|
10
|
100
|
---|
HLA Class II | 625 | 1265 | 690 | 888 | 1109 |
HLA Class I | 93 | 274 | 95 | 85 | 87 |
CD40 | 4 | 7 | 6 | 6 | 7 |
CD80 | 40 | 95 | 57 | 61 | 51 |
CD83 | 5 | 22 | 4 | 5 | 4 |
CD86 | 18 | 71 | 27 | 26 | 33 |
- DC were incubated with paclitaxel at various concentrations or LPS (1 μg/ml) for 2 h before being washed and returned to culture for a further 24 h. Surface expression of various molecules was assessed by flow cytometry as outlined in the materials and methods. The panel reports the delta MFI (the difference between isotype-matched mAb and specific mAb) mean fluorescence intensity (MFI). Data representative of 5 independent experiments.