Figure 4

Percent survival of mice given intraperitoneally (IP) 50 and 5 μg of HuMAbs 5C12, 2F10, 6B7, 6D8 and 6C3 followed 4 h later with IP administration of a lethal Stx2 dose. Mice in PBS control groups died within 2 days of Stx2 challenge. Star (*) above the columns represents significant survival as compared to that of PBS. At 50 μg, 5C12, 2F10 and 6C3 completely protected mice against Stx2-mediated toxicosis and death. At the same dose, the antibodies 6B7 and 6D8 protected 0% and 20% mice, respectively. At the low dose of 5 μg, 5C12 was highly protective since it protected all mice, and 2F10 was moderately protective since it protected 60% of mice. At the same dose, the antibody 6C3 showed low level protection (protected 20% of mice), and the antibodies 6B7 and 6D8 failed to protect any mouse.