Figure 7

Subcellular localization of STAT1 in different cell-types after stimulation with IFN-a1 and IFNγ. The cells were treated with IFN-a1 (10 U/mL) or IFNγ (200 ng/mL) for 1 or 4 h or left unstimulated before fixed in 4% paraformaldehyde and stained for STAT1 (red). Nuclei were stained with DAPI (blue). (A) Salmon adherent head kidney leukocytes. Translocation of STAT1 to the nucleus took place at 1 h and at 4 h after stimulation with IFNγ as indicated by arrows. (B) TO cells. Translocation of STAT1 to the nucleus took place about 1 h after stimulation with IFNγ as indicated by arrows. (C) CHSE-214 cells. No translocation of STAT1 to the nucleus was observed. A control with no α-STAT1 is included for each of the cell types.