Table 1 Primer sequences and their applications in this study.
From: Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)
Primer name | Sequence 5' - 3' | Application |
|---|---|---|
rtSTAT1fw | CACCATGGCCCAGTGGTGCCAGCTGCA | Gene cloning/sequencing |
rtSTAT1rev | CTACTATCAGTTGCAGTCCGAGTCAGGTG | Gene cloning/sequencing |
ssSTAT1 2204 fw | AGTGTTGGACTGGTCCTAAGGA | Isoform detection (PCR) |
ssSTAT 1gap rev | TGAAATTCTTCAGCTAAAAACTCTC | Isoform detection (PCR) |
ssSTAT1fw | CGGGCCCTGTCACTGTTC | qPCR |
ssSTAT1rev | GGCATACAGGGCTGTCTCT | qPCR |
ssSTAT1 probe | ACCACCAAGGAATGTTC | qPCR |