Figure 4

Regulation of candidate reference genes in LPS stimulated monocytes. Shown are mRNA level changes of candidate reference genes in LPS stimulated monocytes compared to non-stimulated cells (LPS from N. meningitides, blue bars, and LPS from E. coli, red bars). Data were normalized to the normalization factor calculated by geNorm based on the genes PPIB, B2M and PPIA (A) and to the geometric mean of the best-combination of reference genes (TBP and B2M) as suggested by Normfinder (B). Statistical significance was assessed by Wilcoxon matched pairs signed ranks test, and results with p < 0.05 are regarded as statistically significant (indicated by asterisks). Paired student's t test was also performed and resulted in similar findings (data not shown).